abstract：:In order to determine the extent of the repertoire of the immunoglobulin light chain V-region locus (Igl-V) in the laboratory rat (Rattus norvegicus), we constructed a specifically primed cDNA library from lipopolysaccharide-stimulated DA strain rat spleens. The library was screened with a rat Igl-C2-specific probe, a...

journal_title：Immunogenetics

authors： Aguilar BA,Gutman GA

更新日期：1992-01-01 00:00:00

abstract：:A contribution of allelic variation of T-cell receptor (Tcr) genes to the immune response has not been studied. Here we report that the presence of insertion-deletion-related polymorphisms (IDRP) of the Tcr beta chain (Tcrb) can be utilized to distinguish the parental origin of the gene complex that undergoes rearrang...

journal_title：Immunogenetics

authors： Seboun E,Joshi N,Hauser SL

更新日期：1992-01-01 00:00:00

abstract：:The polymerase chain reaction was used to isolate clones with class I major histocompatibility complex sequences from fish (carp), amphibian (axolotl), and two species of reptile (lizard and snake). The lizard and snake clones were used to isolate class I cDNA clones. All the sequences showed the expected evolutionary...

journal_title：Immunogenetics

authors： Grossberger D,Parham P

更新日期：1992-01-01 00:00:00

abstract：:In three families with an apparent non-expressed factor B (BF) allele (BF*Q0), advanced methods of isoelectric focusing for the determination of BF F subtypes revealed different hypomorphic BF products (BF QL) with functional hemolytic activity expressed by the assumed BF*Q0 allele. A Taq I and a Msp I restriction fra...

journal_title：Immunogenetics

authors： Siemens I,Brenden M,Mauff G,Abbal M,Du Toit E,Bertrams J,Geserick G

更新日期：1992-01-01 00:00:00

abstract：:To search for host genes for resistance/susceptibility to cancer metastasis, mutation analysis was employed. Ten putative mutants of resistance to lymphoma EL4 and four putative mutants of resistance to sarcoma MCA/77-23 of C57BL/6J (B6) mice were produced. These mutants were designated S (for "survivor") mutants; the...

journal_title：Immunogenetics

authors： Egorov OS,Liu Y,Sargent EE,Drizlikh GI,Egorov IK

更新日期：1992-01-01 00:00:00

abstract：:Immunoglobulin-A deficiency (IgA-D) is the most common human Ig class deficiency with an estimated frequency of approximately 1 in 500 in the Swedish population. We investigated the immunoglobulin heavy chain constant region gene segments (IGHC) in 103 individuals with IgA-D and the immunoglobulin heavy chain variable...

journal_title：Immunogenetics

authors： Olsson PG,Hammarström L,Cox DW,Smith CI

更新日期：1992-01-01 00:00:00

abstract：:To assess the role interleukins and mitogens play in regulating immunoglobulin (Ig) gene expression via the Ig enhancer and promoter, transgenic mice carrying two different Ig gene regulatory regions were generated. One, EmukCAT, contains the Ig heavy chain enhancer (Emu) and the kappa light chain promoter driving the...

journal_title：Immunogenetics

authors： Miller AE,Ennist DL,Ozato K,Westphal H

更新日期：1992-01-01 00:00:00

abstract：:The recombination activating genes RAG-1 and RAG-2 are adjacent genes that act synergistically to activate variable-diversity-joining (V(D)J) recombination. Southern analysis of hybrid cell lines derived from patients with the Wilms tumor-aniridia-genitourinary defects-mental retardation (WAGR) syndrome and from mutag...

journal_title：Immunogenetics

authors： Oettinger MA,Stanger B,Schatz DG,Glaser T,Call K,Housman D,Baltimore D

更新日期：1992-01-01 00:00:00

abstract：:Hepatic granulomatous lesions were induced in mice by a single intraperitoneal injection of 3 mg of disrupted Streptococcus pyogenes cell-wall material. Mice carrying the H-2b or H-2k haplotypes were highly susceptible to the induction and three weeks after the injection produced numerous granulomas. In contrast, mice...

journal_title：Immunogenetics

authors： Chen CY,Cohen SA,Zaleski MB,Albini B

更新日期：1992-01-01 00:00:00

abstract：:The level of HLA-B27 transgene expression on the cell surface is dependent on the host H-2 haplotype. Mice homozygous for the H-2b, H-2f, H-2s, H-2p, H-2r, and H-2k haplotypes express B27 at high levels. An intermediate level of B27 expression is observed in H-2v mice whereas low levels of B27 are expressed in H-2q an...

journal_title：Immunogenetics

authors： Nickerson-Nutter CL,Hogen KL,David CS

更新日期：1992-01-01 00:00:00

abstract：:A cDNA library prepared from lymphocytes of a cow (E98), homozygous at major histocompatibility complex (MHC) loci (BoLA phenotype w10, KN104), was screened with a bovine MHC class I probe. Of the cDNA clones isolated, two, (2.1 and 5.1) were selected and showed divergence at both 5' and 3' termini. E98 DNA was digest...

journal_title：Immunogenetics

authors： Bensaid A,Kaushal A,Baldwin CL,Clevers H,Young JR,Kemp SJ,MacHugh ND,Toye PG,Teale AJ

更新日期：1991-01-01 00:00:00

abstract：:Protein tyrosine phosphorylation is an important regulatory mechanism in cell physiology. While the protein tyrosine kinase (PTKase) family has been extensively studied, only six protein tyrosine phosphatases (PTPases) have been described. By Southern blot analysis, genomic DNA from several different phyla were found ...

journal_title：Immunogenetics

authors： Matthews RJ,Flores E,Thomas ML

更新日期：1991-01-01 00:00:00

abstract：:Although major histocompatibility complex (MHC) class I molecules are, as a rule, highly polymorphic in mammalian species, those of the New World primate Saguinus oedipus (cotton-top tamarin) exhibit limited polymorphism. We have cloned and sequenced twelve MHC class I cDNAs from this species. Since cloned cotton-top ...

journal_title：Immunogenetics

authors： Watkins DI,Garber TL,Chen ZW,Toukatly G,Hughes AL,Letvin NL

更新日期：1991-01-01 00:00:00

abstract：:Mice bearing the H-2w7 haplotype have five C4-related genes, one C4, one Slp, and three C4/Slp hybrid genes. The expression of these five genes in the liver of H-2w7 mice was estimated at the steady state level of their respective mRNA. We have amplified by the polymerase chain reaction (PCR) three regions of the C4/S...

journal_title：Immunogenetics

authors： Huang ZM,Takahashi M,Nonaka M

更新日期：1991-01-01 00:00:00

abstract：:Endemic pemphigus foliaceus (EPF), is an autoimmune disease associated with production of IgG antibodies against epidermal antigens. We have tested 38 patients and 50 control subjects living in endemic areas to investigate whether HLA genes are associated with host factors that determine whether or not exposed individ...

journal_title：Immunogenetics

authors： Moraes JR,Moraes ME,Fernandez-Vina M,Diaz LA,Friedman H,Campbell IT,Alvarez RR,Sampaio SA,Rivitti EA,Stastny P

更新日期：1991-01-01 00:00:00

abstract：:The glycosyl phosphatidylinositol (GPI)-linked antigen recognized by monoclonal antibody (mAb) MEM-102 is expressed on all peripheral blood lymphocytes, both resting and activated. Its properties are very similar to a previously described activation antigen, Blast-1. The amino acid sequence deduced from the structure ...

journal_title：Immunogenetics

authors： Korínek V,Stefanová I,Angelisová P,Hilgert I,Horejsí V

更新日期：1991-01-01 00:00:00

abstract：:The potential value of in vitro cytotoxic T lymphocyte (CTL) assays for predicting the occurrence of graft vs host disease (GVHD) following allogeneic bone marrow transplantation was evaluated in 12 mouse donor-host combinations associated with various degrees of GVHD. These donor-host combinations were selected after...

journal_title：Immunogenetics

authors： Fontaine P,Langlais J,Perreault C

更新日期：1991-01-01 00:00:00

abstract：:Comparison of the rate of synonymous and nonsynonymous nucleotide substitutions suggests that certain regions of the functional H-2 genes, which are part of the mouse major histocompatibility complex (Mhc), are under strong positive selection pressure. Thus far, however, little evidence has been provided for the exist...

journal_title：Immunogenetics

authors： Ritte U,Neufeld E,O'hUigin C,Figueroa F,Klein J

更新日期：1991-01-01 00:00:00

abstract：:In an effort to generate an A.CA mouse expressing Ed, the Ead gene has been introduced into A.CA mice which lack the major histocompatibility complex (MHC) class II E molecule. Flow cytometric analysis shows cell surface expression of the E alpha chain on lymphocytes and macrophages in the transgenic mice. Analysis of...

journal_title：Immunogenetics

authors： Ishikawa S,Chang MY,Diamond B

更新日期：1991-01-01 00:00:00

abstract：:The identification of 19 different HLA-DPB1 sequences implicates the existence of more DP specificities than can be typed for with cellular methods. How many of the DP beta sequences can be specifically recognized by T cells, and which of the polymorphic regions can contribute to the specificity of allorecognition, is...

journal_title：Immunogenetics

authors： de Koster HS,Kenter MJ,D'Amaro J,Luiten RM,Schroeijers WE,Giphart MJ,Termijtelen A

更新日期：1991-01-01 00:00:00

abstract：:Mouse Ly6A and Ly6C cDNA probes were hybridized to total RNA of rat tissues and, as in mouse, the highest level of Ly6-related transcripts was detected in kidney. Therefore, Ly6-related cDNA clones were isolated from a commercial rat kidney cDNA library in lambda gt11. Four of these (RK3, RK6, RK10, and RK11) have bee...

journal_title：Immunogenetics

authors： Friedman S,Palfree RG,Sirlin S,Hämmerling U

更新日期：1990-01-01 00:00:00

abstract：:We have analyzed genomic DNA in a large number of independent examples of five HLA-DQw2-bearing extended haplotypes for their associated subtypes by restriction fragment length polymorphism (RFLP) using DRB, DQA, and DQB probes after Taq I and Pst I digestion and Southern blotting. In addition to three previously desc...

journal_title：Immunogenetics

authors： Yunis JJ,Wescott M,Lechin SJ,Alosco SM,Awdeh Z,Alper CA,Yunis EJ

更新日期：1990-01-01 00:00:00

abstract：:Mouse leukosialin, previously known as the 3E8 antigen, is expressed primarily on cells of the hematopoietic and lymphoid lineages and is shown to be the mouse homologue to the human leukosialin/sialophorin and rat W3/13 molecules. A partial leukosialin cDNA clone was isolated via cross-species hybridization with a po...

journal_title：Immunogenetics

authors： Baecher CM,Dorfman KS,Mattei MG,Frelinger JG

更新日期：1990-01-01 00:00:00

abstract：:We have recently mapped the human FCGR2 gene to chromosome 1 bands q23-q24. In situ hybridization of FCGR2 cDNA with a cell line containing a t(1:19)(q23;p13) derived from a patient with pre-B ALL has allowed a more accurate localization of this gene to chromosome 1 band q23. Furthermore, this study indicated a splitt...

journal_title：Immunogenetics

authors： Webber LM,Garson OM,Tate B,McKenzie IF,Hogarth PM

更新日期：1990-01-01 00:00:00

abstract：:Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1*1401 (DRw14-Dw9) and DRB1*1402 (DRw14-Dw16). The second exon of the DRB1 gene of the novel DRw14 allele (designated DRB1-14c) ...

journal_title：Immunogenetics

authors： Obata F,Ito I,Ito K,Abe A,Ohkubo M,Onda K,Yang YG,Watanabe K,Kashiwagi N

更新日期：1990-01-01 00:00:00

abstract：:Mice bearing the H-2b haplotype are susceptible to the development of experimental autoimmune myasthenia gravis (EAMG), induced by acetylcholine receptor (AChR) autoimmunity. One of the genes influencing EAMG susceptibility has been mapped to the Ab locus of the major histocompatibility complex, and the A beta chain h...

journal_title：Immunogenetics

authors： Christadoss P,David CS,Shenoy M,Keve S

更新日期：1990-01-01 00:00:00

abstract：:A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. Nucleotide sequence analysis of the two intronless genes, HSP70-1 and HSP70-2, has shown that they encode an identical protein pro...

journal_title：Immunogenetics

authors： Milner CM,Campbell RD

更新日期：1990-01-01 00:00:00

abstract：:The ability of local ultraviolet B (UVB) irradiation to impair the induction of dinitrofluorobenzene (DNFB)-specific contact hypersensitivity (CH) in mice has been shown to be genetically determined. We have explored the possibility that the mouse Tnfa and Lps loci are involved. We demonstrate that C3H/HeN (Lpsn) stra...

journal_title：Immunogenetics

authors： Yoshikawa T,Streilein JW

更新日期：1990-01-01 00:00:00

abstract：:Two out of ten Rg-specific antisera tested contain a third antibody specific for the beta chain of C4. Analysis of the beta chains of 66 unrelated individuals by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the epitope detected is located exclusively on the light (L) beta chain. A strong, bu...

journal_title：Immunogenetics

authors： Robson T,Heard RN,Giles CM

更新日期：1989-01-01 00:00:00

abstract：:We investigated the genetic control of heterotopic heart allograft rejection using a family of standard inbred, major histocompatibility complex (MHC)-congenic, and intra-MHC recombinant rat strains. Gene products of the various regions within the rat MHC differed markedly in their capacity to induce rejection. Isolat...

journal_title：Immunogenetics

authors： Klempnauer J,Steiniger B,Lück R,Günther E

更新日期：1989-01-01 00:00:00

abstract：:The evolution of the mouse immunoglobulin heavy chain variable region (Igh-V) locus was investigated by the comprehensive analysis of variable region (Vh) gene family content and restriction fragment polymorphism in the genus Mus. The examination of natural Mus domesticus populations suggests an important role for rec...

journal_title：Immunogenetics

authors： Tutter A,Riblet R

更新日期：1989-01-01 00:00:00

abstract：:The structure of the DRB1*03 gene has been interpreted as the product of a gene conversion event involving a DRB3 gene as donor and resulting in the introduction of two short segments of the DRB3 sequence into the DRB1 locus. The serological counterpart of this double insertion is the TR81 specificity. Consequently, t...

journal_title：Immunogenetics

authors： Sorrentino R,Iannicola C,Botti S,Costanzi S,Tanigaki N,Tosi R

更新日期：1989-01-01 00:00:00

abstract：:The locus for human immunoglobulin heavy chain constant region genes (IgCH) is characterized by a significant frequency of deleted or duplicated haplotypes, due to unequal crossing-over events. Four types of deletions and one duplication have been reported so far. We describe here a molecular study of four cases of Ig...

journal_title：Immunogenetics

authors： Bottaro A,De Marchi M,De Lange G,Boccazzi C,Caldesi F,Gallina R,Carbonara AO

更新日期：1989-01-01 00:00:00

abstract：:Immunogenicity of allogeneic immunoglobulins in mice were studied, measuring the allotype-specific antibody activity by agglutination of allogeneic antibody-coated red blood cells. It was found that the serum from C.B-20 mice (Ighb, BALB/c-congenic) was uniquely immunogenic in BALB/c mice for allotype antibody respons...

journal_title：Immunogenetics

authors： Inagi R,Yoshida T,Isobe K,Nakashima I

更新日期：1989-01-01 00:00:00

abstract：:The human T-cell receptor gamma (TCRG) locus comprises 14 variable genes (TRGV), five joining segments (TRGJ), and two constant region genes (TRGC). The constant gamma 1 gene, TRGC1, consists of three exons, whereas the TRGC2 gene contains four or five exons due to the duplication or triplication of exon 2 and spans 9...

journal_title：Immunogenetics

authors： Buresi C,Ghanem N,Huck S,Lefranc G,Lefranc MP

更新日期：1989-01-01 00:00:00

abstract：:We used rabbit antisera to the chicken MHC erythrocyte molecule B-G and to the class I alpha chain (B-F) to screen lambda gt11 cDNA expression libraries made with RNA selected by oligo-dT from bone marrow cells of anemic B19 homozygous chickens. Eight clones were found to encode B-G molecules which hybridize with sequ...

journal_title：Immunogenetics

authors： Kaufman J,Salomonsen J,Skjødt K

更新日期：1989-01-01 00:00:00

abstract：:The immune response to bovine insulin (BI) in the rat is controlled by the major histocompatibility complex (Mhc)-linked immune response gene (Ir-BI) and immune suppression gene (Is-BI). In the present study, we investigated the low responsiveness to BI in the WKAH rat (RT1k) and attempted to explore the functional li...

journal_title：Immunogenetics

authors： Mizuno K,Tsuchimoto S,Matsuno Y,Niiyama T,Fujii H,Natori T,Aizawa M

更新日期：1988-01-01 00:00:00

abstract：:The major histocompatibility complex (MHC) of the domestic cat has been poorly characterized to date, primarily because of numerous difficulties in the preparation of allotypic sera. We present here a comparative analysis of class I and class II genes in domestic cat populations using molecular probes of the MHC from ...

journal_title：Immunogenetics

authors： Yuhki N,O'Brien SJ

更新日期：1988-01-01 00:00:00

abstract：:The HLA-A2 antigen expressed by donor OZB can be distinguished from the main HLA-A2.1 subtype by isoelectric focusing - it is one charge unit more acidic - and by some alloreactive T-cell clones but not by cytolytic T lymphocyte lines. The structure of variant OZB has been examined by comparative peptide mapping with ...

journal_title：Immunogenetics

authors： Castaño R,Ezquerra A,Doménech N,López de Castro JA

更新日期：1988-01-01 00:00:00

abstract：:The organization of the major histocompatibility complex (MHC) class II region in cattle was investigated by Southern blot analysis using human probes corresponding to DO, DP, DQ, and DR genes. Exon-specific probes were also employed to facilitate the assessment of the number of different bovine class II genes. The re...

journal_title：Immunogenetics

authors： Andersson L,Rask L

更新日期：1988-01-01 00:00:00